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Direct dilution is the process by which Echo Liquid Handlers generates the IC 50 curve, transferring precise droplets of solution directly to individual assay wells, without tips. This straightforward process can generate a 12-point IC 50 curve from a stock solution and two intermediate dilutions, improving reliability and data quality.
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Abstract Open abdomen (OA) has been an effective treatment for abdominal catastrophes in traumatic and general surgery. However, management of patients with OA remains a formidable task for surgeons. The central goal of OA is closure of fascial defect as early as is clinically feasible without precipitating abdominal compartment syndrome. Historically, techniques such as packing, mesh, and vacuum-assisted closure have been developed to assist temporary abdominal closure, and techniques such as components separation, mesh-mediated traction, bridging fascial defect with permanent synthetic mesh, or biologic mesh have also been attempted to achieve early primary fascial closure, either alone or in combined use.
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